Journal: Fluids and barriers of the CNS

Article Title: Transcriptomic analysis of a 3D blood-brain barrier model exposed to disturbed fluid flow.

doi: 10.1186/s12987-022-00389-x

Figure Lengend Snippet: Fig. 2 Functional enrichment analysis compared to the steady, disturbed condition for A pulsatile, disturbed, B static, and C steady, fully developed. D Volcano plot with the location of lumican indicated by LUM

Article Snippet: Lumican siRNA (Santa Cruz, sc-44805) was added to the transfection solution and exposed to the cells for 6 h at 37C.

Techniques: Functional Assay

Journal: Fluids and barriers of the CNS

Article Title: Transcriptomic analysis of a 3D blood-brain barrier model exposed to disturbed fluid flow.

doi: 10.1186/s12987-022-00389-x

Figure Lengend Snippet: Fig. 3 A qRT-PCR quantification of differentially expressed genes identified by RNA sequencing. B Lumican quantification between portions of the vessel exposed to steady and fully developed. C-D Portions of fully developed (C) and disturbed (D) regions from the 3D vessels stained with anti-ZO-1 (red) and anti-lumican (green) and DAPI (blue). Scale bar = 25 μm. E Protein quantification of lumican expression from monolayers exposed to uniform shear stress (“fully developed”) or a shear gradient (“disturbed”). * p < 0.05

Article Snippet: Lumican siRNA (Santa Cruz, sc-44805) was added to the transfection solution and exposed to the cells for 6 h at 37C.

Techniques: Quantitative RT-PCR, RNA Sequencing, Staining, Expressing, Shear

Journal: Fluids and barriers of the CNS

Article Title: Transcriptomic analysis of a 3D blood-brain barrier model exposed to disturbed fluid flow.

doi: 10.1186/s12987-022-00389-x

Figure Lengend Snippet: Fig. 4 A Validation of lumican knockdown efficiency. B-C Immunofluorescence indicating the effect of lumican knockdown (B) on localization of ZO-1 to cell–cell junctions in both fully developed (i) and disturbed (ii) regions, with scrambled siRNA, labeled as "KD" serving as control (C). D Permeability quantification of vessels after 24 h perfusion. Scale bar = 50 μm, * p < 0.05. n = 3 per condition

Article Snippet: Lumican siRNA (Santa Cruz, sc-44805) was added to the transfection solution and exposed to the cells for 6 h at 37C.

Techniques: Biomarker Discovery, Knockdown, Immunofluorescence, Labeling, Control, Permeability

Journal: Fluids and barriers of the CNS

Article Title: Transcriptomic analysis of a 3D blood-brain barrier model exposed to disturbed fluid flow.

doi: 10.1186/s12987-022-00389-x

Figure Lengend Snippet: Fig. 5 A Axial cross-section of the vessel showing the location of GFP-labeled endothelial cells, and smooth muscle and astrocytes stained with anti-alpha-SMA and anti-GFAP, respectively. Scale = 100 μm. B Radial cross-sectional images of the vessel lumens indicating differences in lumican expression between scrambled (i) and knockdown (ii) conditions. Scale bar = 100 μm. C ZO-1 localization to cell–cell junctions of knockdown cells with lumican added exogenously into the matrix in the fully developed (C,i) compared to the disturbed (C,ii) region. Scale bar = 25 μm. D Permeability quantification compared to static controls in fully developed and disturbed regions, * p < 0.05. n = 3 per condition

Article Snippet: Lumican siRNA (Santa Cruz, sc-44805) was added to the transfection solution and exposed to the cells for 6 h at 37C.

Techniques: Labeling, Staining, Expressing, Knockdown, Permeability